This African penguin baby will sadly not be named after a hot dog. Breakthroughs, discoveries, and DIY tips sent every weekday. Last December, staff at Adventure Aquarium in Camden, New Jersey, celebrated the arrival of two newly hatched African penguin chicks (). Their births marked a big moment in conservation efforts for the critically endangered species, but even more good news was apparently on the way. Less than a month after welcoming Duffy and Oscar to the flock, Adventure Aquarium has announced newcomer.

Hospital call centers serve as the primary contact point for patients within a hospital system. They also generate substantial volumes of staff messages as navigators process patient requests and communicate with the hospital offices following the established protocol restrictions and guidelines. This continuously accumulated large amount of text data can be mined and processed to retrieve insights; however, traditional supervised learning approaches require annotated data, extensive training, and model tuning. Large Language Models (LLMs) offer a paradigm shift toward more computationally efficient methodologies for healthcare analytics. This paper presents a multi-stage LLM-based framework that identifies staff message topics and classifies messages by their reasons in a multi-class fashion. In the process, multiple LLM types, including reasoning, general-purpose, and lightweight models, were evaluated. The best-performing model was o3, achieving 78.4% weighted F1-score and 79.2% accuracy, followed closely by gpt-5 (75.3% Weighted F1-score and 76.2% accuracy). The proposed methodology incorporates data security measures and HIPAA compliance requirements essential for healthcare environments. The processed LLM outputs are integrated into a visualization decision support tool that transforms the staff messages into actionable insights accessible to healthcare professionals. This approach enables more efficient utilization of the collected staff messaging data, identifies navigator training opportunities, and supports improved patient experience and care quality.

Millions of patients are already using large language model (LLM) chatbots for medical advice on a regular basis, raising patient safety concerns. This physician-led red-teaming study compares the safety of four publicly available chatbots--Claude by Anthropic, Gemini by Google, GPT-4o by OpenAI, and Llama3-70B by Meta--on a new dataset, HealthAdvice, using an evaluation framework that enables quantitative and qualitative analysis. In total, 888 chatbot responses are evaluated for 222 patient-posed advice-seeking medical questions on primary care topics spanning internal medicine, women's health, and pediatrics. We find statistically significant differences between chatbots. The rate of problematic responses varies from 21.6 percent (Claude) to 43.2 percent (Llama), with unsafe responses varying from 5 percent (Claude) to 13 percent (GPT-4o, Llama). Qualitative results reveal chatbot responses with the potential to lead to serious patient harm. This study suggests that millions of patients could be receiving unsafe medical advice from publicly available chatbots, and further work is needed to improve the clinical safety of these powerful tools.

-- The hippocampus, a critical brain structure involved in memory processing and various neurodegenerative and psychiatric disorders, comprises three key subregions: the dentate gyrus (DG), Cornu Ammonis 1 (CA1), and Cornu Ammonis 3 (CA3). Accurate segmentation of these subregions from histol ogical tissue images is essential for advancing our understanding of disease mechanisms, developmental dynamics, and therapeutic interventions. However, no existing methods address the automated segmentation of hippocampal subregions from tissue images, pa rticularly from immunohistochemistry (IHC) images. To bridge this gap, we introduce a novel set of four comprehensive murine hippocampal IHC datasets featuring distinct staining modalities: cFos, NeuN, and multiplexed stains combining cFos, NeuN, and eithe r ΔFosB or GAD 67, capturing structural, neuronal activity, and plasticity associated information. Additionally, we propose ROIsGAN, a region - guided U - Net - based generative adversarial network tailored for hippocampal subregion segmentation. By leveraging ad versarial learning, ROIsGAN enhances boundary delineation and structural detail refinement through a novel region guided discriminator loss combining Dice and binary cross - entropy loss. Evaluated across DG, CA1, and CA3 subregions, ROIsGAN consistently out performs conventional segmentation models, achieving performance gains ranging from 1 - 10% in Dice score and up to 11% in Intersection over Union (IoU), particularly under challenging staining conditions. Our work establishes foundational datasets and metho ds for automated hippocampal segmentation, enabling scalable, high - precision analysis of tissue images in neuroscience research. I. INTRODUCTION The hippocampus is one of the most extensively studied areas in the brain because of its significant functional role in memory processing, its remarkable plasticity, and its involvement in This paper is submitted for review on May 13, 2025. Sayed Mehedi Azim is with the Center for Computational and Integrative Biology, Rutgers University, Camden, NJ 18103, USA (e - mail: sayedmehedi.azim@rutgers.edu).

Adversarial noise introduces small perturbations in images, misleading deep learning models into misclassification and significantly impacting recognition accuracy. In this study, we analyzed the effects of Fast Gradient Sign Method (FGSM) adversarial noise on image classification and investigated whether training on specific image features can improve robustness. We hypothesize that while adversarial noise perturbs various regions of an image, edges may remain relatively stable and provide essential structural information for classification. To test this, we conducted a series of experiments using brain tumor and COVID datasets. Initially, we trained the models on clean images and then introduced subtle adversarial perturbations, which caused deep learning models to significantly misclassify the images. Retraining on a combination of clean and noisy images led to improved performance. To evaluate the robustness of the edge features, we extracted edges from the original/clean images and trained the models exclusively on edge-based representations. When noise was introduced to the images, the edge-based models demonstrated greater resilience to adversarial attacks compared to those trained on the original or clean images. These results suggest that while adversarial noise is able to exploit complex non-edge regions significantly more than edges, the improvement in the accuracy after retraining is marginally more in the original data as compared to the edges. Thus, leveraging edge-based learning can improve the resilience of deep learning models against adversarial perturbations.

Precise cell classification is essential in biomedical diagnostics and therapeutic monitoring, particularly for identifying diverse cell types involved in various diseases. Traditional cell classification methods such as flow cytometry depend on molecular labeling which is often costly, time-intensive, and can alter cell integrity. To overcome these limitations, we present a label-free machine learning framework for cell classification, designed for real-time sorting applications using bright-field microscopy images. This approach leverages a teacher-student model architecture enhanced by knowledge distillation, achieving high efficiency and scalability across different cell types. Demonstrated through a use case of classifying lymphocyte subsets, our framework accurately classifies T4, T8, and B cell types with a dataset of 80,000 preprocessed images, accessible via an open-source Python package for easy adaptation. Our teacher model attained 98\% accuracy in differentiating T4 cells from B cells and 93\% accuracy in zero-shot classification between T8 and B cells. Remarkably, our student model operates with only 0.02\% of the teacher model's parameters, enabling field-programmable gate array (FPGA) deployment. Our FPGA-accelerated student model achieves an ultra-low inference latency of just 14.5~$\mu$s and a complete cell detection-to-sorting trigger time of 24.7~$\mu$s, delivering 12x and 40x improvements over the previous state-of-the-art real-time cell analysis algorithm in inference and total latency, respectively, while preserving accuracy comparable to the teacher model. This framework provides a scalable, cost-effective solution for lymphocyte classification, as well as a new SOTA real-time cell sorting implementation for rapid identification of subsets using in situ deep learning on off-the-shelf computing hardware.

Patient experience and care quality are crucial for a hospital's sustainability and reputation. The analysis of patient feedback offers valuable insight into patient satisfaction and outcomes. However, the unstructured nature of these comments poses challenges for traditional machine learning methods following a supervised learning paradigm. This is due to the unavailability of labeled data and the nuances these texts encompass. This research explores leveraging Large Language Models (LLMs) in conducting Multi-label Text Classification (MLTC) of inpatient comments shared after a stay in the hospital. GPT-4 Turbo was leveraged to conduct the classification. However, given the sensitive nature of patients' comments, a security layer is introduced before feeding the data to the LLM through a Protected Health Information (PHI) detection framework, which ensures patients' de-identification. Additionally, using the prompt engineering framework, zero-shot learning, in-context learning, and chain-of-thought prompting were experimented with. Results demonstrate that GPT-4 Turbo, whether following a zero-shot or few-shot setting, outperforms traditional methods and Pre-trained Language Models (PLMs) and achieves the highest overall performance with an F1-score of 76.12% and a weighted F1-score of 73.61% followed closely by the few-shot learning results. Subsequently, the results' association with other patient experience structured variables (e.g., rating) was conducted. The study enhances MLTC through the application of LLMs, offering healthcare practitioners an efficient method to gain deeper insights into patient feedback and deliver prompt, appropriate responses.

In this article, we explore the technical details of the reinforcement learning (RL) algorithms that were deployed in the largest field test of automated vehicles designed to smooth traffic flow in history as of 2023, uncovering the challenges and breakthroughs that come with developing RL controllers for automated vehicles. We delve into the fundamental concepts behind RL algorithms and their application in the context of self-driving cars, discussing the developmental process from simulation to deployment in detail, from designing simulators to reward function shaping. We present the results in both simulation and deployment, discussing the flow-smoothing benefits of the RL controller. From understanding the basics of Markov decision processes to exploring advanced techniques such as deep RL, our article offers a comprehensive overview and deep dive of the theoretical foundations and practical implementations driving this rapidly evolving field. We also showcase real-world case studies and alternative research projects that highlight the impact of RL controllers in revolutionizing autonomous driving. From tackling complex urban environments to dealing with unpredictable traffic scenarios, these intelligent controllers are pushing the boundaries of what automated vehicles can achieve. Furthermore, we examine the safety considerations and hardware-focused technical details surrounding deployment of RL controllers into automated vehicles. As these algorithms learn and evolve through interactions with the environment, ensuring their behavior aligns with safety standards becomes crucial. We explore the methodologies and frameworks being developed to address these challenges, emphasizing the importance of building reliable control systems for automated vehicles.

This study investigated the performance, explainability, and robustness of deployed artificial intelligence (AI) models in predicting mortality during the COVID-19 pandemic and beyond. The first study of its kind, we found that Bayesian Neural Networks (BNNs) and intelligent training techniques allowed our models to maintain performance amidst significant data shifts. Our results emphasize the importance of developing robust AI models capable of matching or surpassing clinician predictions, even under challenging conditions. Our exploration of model explainability revealed that stochastic models generate more diverse and personalized explanations thereby highlighting the need for AI models that provide detailed and individualized insights in real-world clinical settings. Furthermore, we underscored the importance of quantifying uncertainty in AI models which enables clinicians to make better-informed decisions based on reliable predictions. Our study advocates for prioritizing implementation science in AI research for healthcare and ensuring that AI solutions are practical, beneficial, and sustainable in real-world clinical environments. By addressing unique challenges and complexities in healthcare settings, researchers can develop AI models that effectively improve clinical practice and patient outcomes.

The human genome encodes a family of editing enzymes known as APOBEC3 (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3). Several family members, such as APO-BEC3G, APOBEC3F, and APOBEC3H haplotype II, exhibit activity against viruses such as HIV. These enzymes induce C-to-U mutations in the negative strand of viral genomes, resulting in multiple G-to-A changes, commonly referred to as 'hypermutation.' Mutations catalyzed by these enzymes are sequence context-dependent in the HIV genome; for instance, APOBEC3G preferen-tially mutates G within GG, TGG, and TGGG contexts, while other members mutate G within GA, TGA, and TGAA contexts. However, the same sequence context has not been explored in relation to these enzymes and HBV. In this study, our objective is to identify the mutational footprint of APOBEC3 enzymes in the HBV genome. To achieve this, we employ a multivariable data analytics technique to investigate motif preferences and potential sequence hierarchies of mutation by APOBEC3 enzymes using full genome HBV sequences from a diverse range of naturally infected patients. This approach allows us to distinguish between normal and hypermutated sequences based on the representation of mono- to tetra-nucleotide motifs. Additionally, we aim to identify motifs associated with hypermutation induced by different APOBEC3 enzymes in HBV genomes. Our analyses reveal that either APOBEC3 enzymes are not active against HBV, or the induction of G-to-A mutations by these enzymes is not sequence context-dependent in the HBV genome.